Dosage: Vaccinate healthy dogs 3 weeks of age or older. Standard dose is 1 ml administered intranasally. Vaccine may be rehydrated with less than 1 ml of diluent to minimize discomfort when vaccinating small dogs and puppies. For one 1 ml dose, rehydrate vaccine with 1 ml sterile diluent; or for one 0.5 ml dose, rehydrate vaccine with 0.5 ml sterile diluent. Instill the rehydrated vaccine into one or both nostrils of dogs. Annual revaccination with one dose is recommended. Warnings: Contains penicillin, streptomycin, and nystatin as preservatives. This product is designed for intranasal use only with the enclosed applicators. Systemic reactions resulting from inadvertent intramuscular or subcutaneous injection have been reported. Symptoms may include vomiting, diarrhea, lethargy, inappetence, jaundice and death associated with liver failure. Localized tissue necrosis at the injection site has also been reported. If inadvertent injection occurs, monitor the dog closely. Supportive therapy including IV fluids and treatment with gentamicin, tetracycline, trimethoprim/sulfa, or amoxicillin/clavulanic acid may be indicated. If anaphylactoid reaction occurs, use epinephrine. Post-vaccinal reactions consisting of mild canine cough syndrome may occur following use of this vaccine. Nobivac vaccines may experience "Meltback" which can cause some discoloration to the cake (dry) portion of the vaccine. This is purely cosmetic and does not affect the efficacy of the vaccination.